乳酸菌（lactic acid bacteria，LAB）作为一种益生菌，大多数为厌氧菌，多在肠道中存在，本实验以健康犬粪便为材料，用MRS固体培养基进行厌氧培养，对分离得到的菌株进行革兰氏染色，并针对乳酸菌的鉴定设计5个生化试验，分别为触酶试验、吲哚靛基质试验、硝酸盐还原试验、硫化氢试验、明胶液化试验，最后对分离得到的菌株进行16S rDNA测序。本实验通过分离纯化得到4株乳酸菌，菌株全为革兰氏阳性菌，并且形状不同，有球状、片装、明串珠状、双球状四种形态不同的菌株，生化试验结果均为阴性，通过测序确定分离得到的乳酸菌株有３种，分别为肠球菌（2株），片球菌（1株），明串珠菌（1株）,为开发新型的微生态制剂和乳酸菌新型疫苗奠定基础。

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Lactic acid bacteria, lab, as a probiotic, mostly anaerobic bacteria, In the intestinal tract, the laboratory experiments were made with a healthy dog dung, which was used as a form of something Growth medium for the use of mrs solid. For the isolated strains of the strain, and the testing of the lactic acid bacteria, five biochemical tests were designed to Gram staining Testing of something, something project, nitrate restoration, hydrogen sulphide test, gelatin test, Finally, a 16-something sequencing for the isolated strain. The experiment was separated from pure something by four lactic acid bacteria, and the strain was all Gram-positive bacteria and different shapes. With spheres, something, beads of beads, four different types of something, the results are negative. By sequencing certain types of lactic acid strains that are isolated, there are two strains of Enterococcus, two in coccus, one in a series of beads, To develop new types of micro-organisms and lactic acid bacteria new vaccines