调节少突胶质前体细胞发育的转铁蛋白受体及铁蛋白重链
Transferrin receptor and
ferritin-H are developmentally regulated in oligodendrocyte lineage cells
摘要
铁对于细胞存活及分化非常重要，尤其是少突胶质细胞，需要相对高的摄入以制造髓鞘。在脑内有多个铁代谢相关蛋白的表达，目前对它们在少突胶质细胞发育过程中的变化尚不明确。实验观察体外增殖和诱导分化条件培养下的少突胶质细胞模型发现，转铁蛋白受体和铁蛋白重链均在少突胶质细胞的发育过程中表达增加，说明铁在少突胶质细胞发育起重要作用。在培养基中填加不同剂量的Fe3+并没有影响少突胶质前体细胞的成熟及铁蛋白重链的表达，但转铁蛋白受体表达下降。提示转铁蛋白受体和铁蛋白重链表达上调可能促进少突胶质前体细胞的成熟及髓鞘化。
关键词： 神经再生； 神经发生； 少突胶质细胞； 铁离子； 转铁蛋白受体； 铁蛋白重链； 发育； 诱导分化； 基金资助文章； 图片文章；
Abstract:
Iron is an essential trophic element
that is required for cell viability and differentiation, especially in
oligodendrocytes, which consume relatively high rates of energy to produce
myelin. Multiple iron metabolism proteins are expressed in the brain
including transferrin receptor and ferritin-H. However, it is still unknown
whether they are developmentally regulated in oligodendrocyte lineage cells
for myelination. Here, using an in vitro cultured differentiation model of
oligodendrocytes, we found that both transferrin receptor and ferritin-H are
significantly upregulated during oligodendrocyte maturation, implying the
essential role of iron in the development of oligodendrocytes. Additional
different doses of Fe3+ in the cultured medium did not affect
oligodendrocyte precursor cell maturation or ferritin-H expression but
decreased the expression of the transferrin receptor. These results indicate
that upregulation of both transferrin receptor and ferritin-H contributes to
maturation and myelination of oligodendrocyte precursor cells.
Key words： neural regeneration; neurogenesis, oligodendrocyte; iron; transferrin receptor; ferritin-H; development; myelinization; proliferation;induced differentiation; grants-supported paper; photographs-containing paper; neuroregeneration;
研究亮点：
(1) Transferrin
receptor and ferritin-H are expressed in oligodendrocyte lineage cells.
(2) Transferrin receptor and ferritin-H are upregulated during maturation of
oligodendrocyte lineage cells.
(3) Additional iron does not affect oligodendrocyte precursor cell maturation
or ferritin-H expression but decreases the expression of transferrin receptor.
1.少突胶质细胞表达转铁蛋白受体和铁蛋白重链。
2.在少突胶质细胞成熟过程中，转铁蛋白受体和铁蛋白重链表达增加。
3.额外的铁不影响少突胶质前体细胞的成熟，不影响少突胶质前体细胞中铁蛋白重链的表达，但降低了转铁蛋白受体的表达。